Abstract. The hypothesis that ozonated oil has wound healing property was investigated in an excision wound model in rats. The animals were divided into groups which were treated with sesame oil (vehicle), framycetin (standard), or two doses of ozonated sesame oil, topically applied on the excision wounds once daily for 11 consecutive days; the animals were euthanized on the 12th day. Wound healing was assessed by measuring the wound contracture, tensile strength, collagen content and superoxide dismutase activity of skin of the excised wound area.
Excision wound model in rats simulates one of the most commonly encountered wounds in day to day life like abrasions. Framycetin sulphate was chosen as a standard drug for comparison because it is one of the most commonly used antibacterial drug for treating wounds.
The wound contracting ability of high dose of ozonated sesame oil was significantly greater than that of vehicle control and also greater than that of framycetin. Low dose ozonated sesame oil did not have a significant effect on wound contracture when compared with vehicle control.
The tensile strength of both high and low doses of ozonated sesame oil treated wounds were significantly greater than that of vehicle control, and greater than that of framycetin treated wounds.
The impact of the different treatments on collagen deposition correlated with the corresponding tensile strengths of the wounds: collagen content of high and low dose ozonated sesame oil were significantly greater than that of vehicle control and higher than that of framycetin treated wounds.
The activity of the antioxidant enzyme SOD in wounds of high dose of ozonated sesame oil was significantly greater than that of vehicle control, and greater when compared with framycetin.
Histopathological studies revealed that the quality of wound healing in ozonated oil treated groups was better than the vehicle treated group, and the histopathological features of high dose ozonated oil were similar to framycetin treated wounds.
Higher degree of ozonation results in better wound healing properties. Ozone could be a part of the wound care armamentarium not only by virtue of its antibacterial activity, but also due to its antioxidant potential. It is necessary to titrate the dose of ozone in order to gain the maximum benefit of a traditional wound care agent.